Phosphatidylcholine metabolism in neonatal mouse calvaria

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Phosphatidylcholine metabolism in neonatal mouse calvaria.

Phosphatidylcholine metabolism was examined in neonatal mouse calvaria in vitro. Incorporation of choline into phosphatidylcholine was slow in this tissue. At 2 h after a pulse of [methyl-3H]choline only 30% of the tissue radioactivity was in the organic phase. Chromatography of the aqueous phase of the tissue extract revealed that more than half of the radioactivity was present as choline at t...

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Cyclosporine A inhibits bone resorption in cultured neonatal mouse calvaria.

The effect of the immunosuppressive agent cyclosporine A (CsA) on the resorption of neonatal mouse calvaria was examined in vitro. CsA, at concentrations of 10(-7) to 3 X 10(-5) M, inhibited bone resorption produced by 10(-8) M parathyroid hormone, 3 U/ml of mouse recombinant interleukin-1,5 X 10(-7) M prostaglandin E2, 14 U/ml of thrombin, 5 micrograms/ml of bacterial lipopolysaccharide or 10(...

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lysosomal-enzyme release in cultured mouse calvaria

The effect of bradykinin on bone resorption was studied in cultures of newbornmouse calvaria. Bradykinin (0.03,UM, 1 Mm) stimulated the release of 4'Ca2+ from bones dissected out from mice prelabelled in vivo with 45Ca. Bradykinin (1 Mm) also augmented the release of stable calcium (4"Ca), Pi and the lysosomal enzyme f,glucuronidase. The stimulatory effect of bradykinin on mineral mobilization ...

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Glucocorticoid-induced Alterations in Phosphatidylcholine Metabolism in Mouse Lymphoma Cells, L5178Y, in Vitro1

the absence of the steroid. In both, controls as well as hormone-treated cultures, Chromatographie separation of the acid-soluble fraction revealed that 95 to 98% of the radioactivity was associated with phosphorylcholine and that only a small amount was present as either cytidine diphosphate choline or choline. The labeled material in the lipid fraction consisted mainly of phosphatidylcholine ...

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Glucocorticoid-induced alterations in phosphatidylcholine metabolism in mouse lymphoma cells, L5178Y, in vitro.

the absence of the steroid. In both, controls as well as hormone-treated cultures, Chromatographie separation of the acid-soluble fraction revealed that 95 to 98% of the radioactivity was associated with phosphorylcholine and that only a small amount was present as either cytidine diphosphate choline or choline. The labeled material in the lipid fraction consisted mainly of phosphatidylcholine ...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1987

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj2440409